Like a 4 count or whatever time sig click to start the song. Is it possible to do in EOF or does it need to be added to the audio file?

As far as I know, you can still use "Beat>Rocksmith>Place RS Event" to place a high/low pitched tick on beat markers. It's worth a try.

@@raynebc and it works as intended every single time i've used it (and i do it a lot).

 

Just a side note to that, the metronome sound is linked to the sound effect slider in the mixer, so depending on people settings, they might not hear it.

That's a good point. If you want to force the player to hear it regardless of SFX volume settings, you'll have to edit the chart audio. Supposedly Audacity has a "Generate>Click track" feature, but I've never used it.